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plasmid paav cmv zsgreen  (TaKaRa)


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    TaKaRa plasmid paav cmv zsgreen
    Plasmid Paav Cmv Zsgreen, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+vector/pAAV-ZsGreen1+Vector/us12600969-703-1-6
    Average 94 stars, based on 61 article reviews
    plasmid paav cmv zsgreen - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Modeling Reduced Contractility and Stiffness Using iPSC-Derived Cardiomyocytes Generated From Female Becker Muscular Dystrophy Carrier
    Article Snippet: After PCR, each generated droplets were individually detected for fluorescence and analyzed by a QX200 droplet reader (Bio-Rad). coding sequence was cloned from human cDNA using PCR primers and the B4GALT1-EGFP sequence was then subcloned into a pAAV cytomegalovirus (CMV) vector. .. HEK293T cells were transfected with pAAV vector, pHelper vector, and a pRC2-mi342 vector (AAVpro Helper Free System, TaKaRa) using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, TaKaRa) according to the manufacture’s protocol. ..

    Plasmid Preparation:

    Article Title: Modeling Reduced Contractility and Stiffness Using iPSC-Derived Cardiomyocytes Generated From Female Becker Muscular Dystrophy Carrier
    Article Snippet: After PCR, each generated droplets were individually detected for fluorescence and analyzed by a QX200 droplet reader (Bio-Rad). coding sequence was cloned from human cDNA using PCR primers and the B4GALT1-EGFP sequence was then subcloned into a pAAV cytomegalovirus (CMV) vector. .. HEK293T cells were transfected with pAAV vector, pHelper vector, and a pRC2-mi342 vector (AAVpro Helper Free System, TaKaRa) using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, TaKaRa) according to the manufacture’s protocol. ..

    Article Title: Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate HDR template. hU6 promoter and downstream single guide RNA (sgRNA) sequence was amplified from pX459 vector (Addgene #48139). hU6 promoter sequence, sgRNA sequence and HDR template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: Liver-specific inducible promoters and methods of use thereof
    Article Snippet: .. To facilitate this the two aforementioned constructs were cloned into pAAV vector (Takara, Clontech) to allow for the preparation of AAV viruses. ..

    Article Title: Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30,530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate repair template. hU6 promoter and downstream guide RNA (gRNA) sequence was amplified from pX459 vector (Addgene #48,139). hU6 promoter sequence, gRNA sequence and repair template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Clone Assay:

    Article Title: Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate HDR template. hU6 promoter and downstream single guide RNA (sgRNA) sequence was amplified from pX459 vector (Addgene #48139). hU6 promoter sequence, sgRNA sequence and HDR template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: Liver-specific inducible promoters and methods of use thereof
    Article Snippet: .. To facilitate this the two aforementioned constructs were cloned into pAAV vector (Takara, Clontech) to allow for the preparation of AAV viruses. ..

    Article Title: Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30,530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate repair template. hU6 promoter and downstream guide RNA (gRNA) sequence was amplified from pX459 vector (Addgene #48,139). hU6 promoter sequence, gRNA sequence and repair template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Sequencing:

    Article Title: Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate HDR template. hU6 promoter and downstream single guide RNA (sgRNA) sequence was amplified from pX459 vector (Addgene #48139). hU6 promoter sequence, sgRNA sequence and HDR template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Article Title: Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30,530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate repair template. hU6 promoter and downstream guide RNA (gRNA) sequence was amplified from pX459 vector (Addgene #48,139). hU6 promoter sequence, gRNA sequence and repair template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Amplification:

    Article Title: Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate HDR template. hU6 promoter and downstream single guide RNA (sgRNA) sequence was amplified from pX459 vector (Addgene #48139). hU6 promoter sequence, sgRNA sequence and HDR template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes
    Article Snippet: DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30,530). .. Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate repair template. hU6 promoter and downstream guide RNA (gRNA) sequence was amplified from pX459 vector (Addgene #48,139). hU6 promoter sequence, gRNA sequence and repair template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). ..

    Polymerase Chain Reaction:

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Knock-In:

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Cloning:

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: .. PCR fragments of the amplified arms of homology and cloned knock-in sequences and the pAAV vector were seamlessly integrated into a single vector by In-Fusion cloning technology (#639648, Takara Bio). .. All plasmids were cloned and prepared in large quantities using E. coli DH5α (#9057, Takara Bio).

    Construct:

    Article Title: Liver-specific inducible promoters and methods of use thereof
    Article Snippet: .. To facilitate this the two aforementioned constructs were cloned into pAAV vector (Takara, Clontech) to allow for the preparation of AAV viruses. ..

    Bioprocessing:

    Article Title: Liver-specific inducible promoters and methods of use thereof
    Article Snippet: .. To facilitate this the two aforementioned constructs were cloned into pAAV vector (Takara, Clontech) to allow for the preparation of AAV viruses. ..



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    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections <t>of</t> <t>Cre-dependent</t> <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
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    Addgene inc cre expression vector paav tbg pi cre
    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or <t>AAV8-hSyn-DIO-hM3D(Gq)-mCherry,</t> with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
    Cre Expression Vector Paav Tbg Pi Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc egfp vector control
    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or <t>AAV8-hSyn-DIO-hM3D(Gq)-mCherry,</t> with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
    Egfp Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc cre dependent aav5 vector encoding channelrhodopsin paav ef1a double floxed hchr2 h134r eyfp wpre hghpa
    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent <t>AAV8-hSyn-DIO-hM4D(Gi)-mCherry</t> or <t>AAV8-hSyn-DIO-hM3D(Gq)-mCherry,</t> with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.
    Cre Dependent Aav5 Vector Encoding Channelrhodopsin Paav Ef1a Double Floxed Hchr2 H134r Eyfp Wpre Hghpa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Journal: bioRxiv

    Article Title: External Globus Pallidus Arkypallidal Circuit Dynamics Gate Risk-Taking Behavior

    doi: 10.64898/2026.03.20.713182

    Figure Lengend Snippet: (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Article Snippet: The Gi/o-coupled DREADD vector AAV8-hSyn-DIO-hM4D(Gi)-mCherry (plasmid #44362) and the Gq-coupled DREADD vector AAV8-hSyn-DIO-hM3D(Gq)-mCherry (plasmid #44361) were obtained from Addgene.

    Techniques: Control

    (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Journal: bioRxiv

    Article Title: External Globus Pallidus Arkypallidal Circuit Dynamics Gate Risk-Taking Behavior

    doi: 10.64898/2026.03.20.713182

    Figure Lengend Snippet: (a) Experimental diagram. Npas1-Cre-TdTomato mice received bilateral GPe injections of Cre-dependent AAV8-hSyn-DIO-hM4D(Gi)-mCherry or AAV8-hSyn-DIO-hM3D(Gq)-mCherry, with Cre-negative littermates serving as controls. All animals received C21 prior to testing, ensuring equivalent drug exposure across groups. Mice were tested on the elevated plus maze (EPM) 5 weeks post-surgery. Behavioral sessions were video-recorded for subsequent analysis. (b) Time spent in closed arms, center, and open arms during the EPM shows the expected preference for closed arms across all groups, with no effect of GPe NPAS1 manipulation on arm occupancy (two-way mixed ANOVA, maze compartment F (2,52) = 122.8, p = 2.003e-20; group F (2,26) = 1.546, p = 0.2319). (c) Number of open-arm entries does not differ across groups, indicating no effect of GPe NPAS1 manipulation on exploration of these areas (ANOVA, group F (2,26) = 1.048, p = 0.365). (d) Percent time spent in the open arms in the EPM is comparable across control, hM4D(Gi), and hM3D(Gq) mice, consistent with preserved global EPM performance (ANOVA, group F (2,55) = 0.4928, p = 0.6136). (e) Empirical cumulative distribution functions (ECDFs) of frame-wise horizontal movement in the open arms during the EPM show highly overlapping movement distributions across groups, illustrating the absence of gross shifts in locomotor behavior within high-risk regions of the maze. Pairwise Kolmogorov–Smirnov tests detected statistically significant but very small distributional differences (KS D = 0.02–0.05; FDR-corrected p < 0.001), consistent with negligible effect sizes that do not reflect meaningful differences in open-arm movement dynamics. (f) Distance traveled (two-way mixed ANOVA, group x time F (238,3094) = 0.8741, p = 0.9131), (g) speed (two-way mixed ANOVA, group x time F (238,3094) = 0.8742, p = 0.9129), and (h) acceleration (two-way mixed ANOVA, group x time F (238,3094) = 1.037, p = 0.3419) over time are comparable across control, hM4D(Gi), and hM3D(Gq) mice, indicating preserved global locomotor output across the session. Frame-wise polar histograms of heading direction during EPM show (i) control mice exhibit a modest but significant preference for a closed-arm-oriented heading (Rayleigh test, r = 0.005399, p = 0.001871). (j) hM4D(Gi) mice show a statistically significant, strong preferred closed arm heading (Rayleigh test, r = 0.01707, p = 1.225e-16). (k) In contrast, GPe NPAS1 hM4D(Gq) mice do not exhibit a statistically significant preferred heading orientation (Rayleigh test, r = 0.001264, p = 0.7505). (l) Pose features extracted from video tracking show bound box area across time was decreased for hM3D(Gq) mice compared to control mice across all EPM areas (two-way mixed ANOVA, group x time F (238,3094) = 1.180, p = 0.03497; post hoc control v hM3D(Gq) p = 0.03348). (m) Similarly, box aspect ratio over time was decreased for hM3D(Gq) mice compared to control mice (two-way mixed ANOVA, group x time F (238,3094) = 1.181, p = 0.03486; post hoc control v hM3D(Gq) p = 0.04464). (n) There were no group differences in the change in aspect ratio across time (two-way mixed ANOVA, group x time F (238,3094) = 0.08639, p = 0.06231). Dots represent individual data points, error bars or shaded bands represent standard error of the mean (SEM). For polar plots, 32 bins were computed to generate 11.25 degree bars for histogram densities.

    Article Snippet: The Gi/o-coupled DREADD vector AAV8-hSyn-DIO-hM4D(Gi)-mCherry (plasmid #44362) and the Gq-coupled DREADD vector AAV8-hSyn-DIO-hM3D(Gq)-mCherry (plasmid #44361) were obtained from Addgene.

    Techniques: Control